MABIO4710 Advanced Bioanalytical Methods Course description

The course will give the students an in-depth introduction to key methods for sample preparation and analysis of both exogen and endogen compounds in biological samples. Method development for chromatographic and mass spectrometry analyses will be central to the course. The students will also receive training in the operation and use of advanced analytical instruments that are currently used in qualitative and quantitative studies.

Analytical chemistry/bioanalysis at bachelor’s level.

The course forms a theoretical and practical basis for assessing choices and methods that are relevant both in current and future medical diagnostics and research. This comprises immunological, biochemical, cell and molecular biology methods. A selection of these methods are used in the practical work.

Students must have been admitted to the Master’s Programme in Health and Technology - Specialisation in Biomedicine.

After completing the course, the student should have the following learning outcomes defined in terms of knowledge, skills and general competence:

Knowledge

The student

• understands and is capable of explaining a range of biochemical and cellular biological methods, their possibilities and limitations
• understands the term proteomics, the principle behind selected analytical methods and their applications
• can describe different advanced microscopy techniques, principles and areas of application
• understands the principle behind different methods of preparation of transgenic animals, and can explain the laws and regulations that regulate the production of such animals

Skills

The student

• can assess the suitability of selected biochemical and cell biological methods and their strengths and weaknesses in an independent manner
• can use and critically interpret results from selected methods in the field of biochemistry and cell biology in an independent manner
• can conduct bio-informatic searches in web-based sequence databases
• can present research results in a scientific manner

General competence

The student

• can critically assess relevant methods in biomedical research and diagnostics
• can design an experiment in biochemistry and cell biology

The following must have been approved in order for the student to take the exam:

• attendance at the course introduction
• minimum attendance of 80% at seminars
• minimum attendance of 80% at laboratory courses
• individual lab report based on specified criteria.

Simple handheld calculator.

Grade scale A-F

All answers are assessed by two examiners. An external examiner is used regularly, at a minimum of every third completion of the course. When selecting answers for external evaluation, a minimum of 10 percent of the answers shall be included, with no fewer than 5 answers. The external examiner’s assessment of the selected answers shall benefit all students.

10 credits overlap with MABIO4200 Biochemical and Cell Biological Methods.

None.