KJVE3510 Analytical Chemistry II Course description

The students shall acquire in-depth knowledge of important instrumental analysis methods, sampling and sample preparation in analytical chemistry. The students shall also receive training in principles for method development and method validation, quality control and quality assurance in a chemical laboratory.

Language of instruction: Norwegian

The course builds on KJPE1300 General Chemistry, KJFP1400 Organic Chemistry, KJM1500 Physical Chemistry, and KJM2300 Analytical Chemistry I. It is recommended that students taking the course have passed the exam in KJM2300 Analytical Chemistry I.

Approved laboratory course in KJTS2100 Chemical Engineering, KJM2300 Analytical Chemistry I and KJM2400 Biochemistry or corresponding qualifications.

After completing the course, the student is expected to have achieved the following learning outcomes defined in terms of knowledge, skills and general competence:

Knowledge

The student is capable of explaining:

• theoretical and practical principles for the use of chromatographic techniques, with the emphasis on gas chromatography and liquid chromatography
• the principles of targeted and non-targeted analysis
• the theory, functioning and applications of mass spectrometry, basic interpretation of mass spectra
• general sampling principles and practical techniques for sampling gas, liquid, solids and biological materials
• important sample preparation techniques for chromatographic and mass spectrometric analysis, including extraction, preparative chromatography, protein isolation, ultrafiltration and concentration techniques
• principles for method development, optimization and method validation for chromatography
• principles for quality control and quality assurance in a chemical laboratory
• the importance of planning and preparing laboratory work.
• data processing and analysis of large data sets in analytical chemistry

Skills

The student is capable of:

• preparing various types of sample materials for qualitative and quantitative analysis
• performing quantitative analyses in accordance with standardized operating procedures
• calibrating and adjusting common measurement instruments
• preparing calibration standards and generating calibration curves that satisfy common quality requirements
• choosing the appropriate laboratory equipment and using it correctly
• making their own assessment in the choice of sample preparation and analysis techniques
• reading scientific articles and searching in journal databases to find relevant literature in connection with method development and implementation
• using statistical methods and relevant software for data extraction, data analysis and interpretation and quality assurance in analytical chemistry
• determining the identity of unknown analytes based on chromatographic and mass spectrometry analyses

General competence

The student:

• has insight into the application and limitations of common chromatographic and mass spectrometry analysis techniques
• has insight into requirements for good laboratory practice
• has knowledge of the principles for developing new analysis methods, including sampling, sample preparation, qualitative and quantitative analysis
• can handle sample materials, including biological samples, in an appropriate and safe manner.
• has knowledge of how accuracy and precision in measurement results are affected by sources of error and uncertainty in instrumentation, procedures and work techniques
• has a background for understanding developments and future perspectives in the field
• is capable of communicating their own results orally and in writing

The teaching is organised as lectures, exercises and laboratory instruction.

The following coursework is compulsory and must be approved before the student can sit the exam:

• Six-day laboratory course with 2 written assignments (groups of 2-4 students, 10-15 pages per assignment).
• Before a laboratory exercise can start up, a tutorial must be completed where the students groups submit a written plan on how to carry out the exercise.
• Some exercise sessions related to the laboratory course will be compulsory. These sessions will be announced separately.
• A case study assignment in which the analytical procedure for the practical workshop in bioanalysis is developed by the students (4-6 students per group). The procedure must be approved before the start of the workshop (a written procedure, 6-8 pages).
• One-day practical workshop in bioanalytical metods (4 h laboratory work, groups of 4-6 students, no written report).
• A written collaboration assignment in the form of a case study (groups of 2-4 students). Oral presentation of the solution to the case assignment (6-8 hours). The presentation is to be given in English.

Individual written exam under supervision, 3 hours

The exam result can be appealed.

In the event of a resit or rescheduled exam, oral examination may be used instead of written. If oral exams are used for resit and rescheduled exams, the exam result cannot be appealed.

Excel is made avaliable during the exam. A handheld calculator that cannot be used for wireless communication or to perform symbolic calculations. If the calculator's internal memory can store data, the memory must be deleted before the exam. Random checks may be carried out.

Grade scale A-F.

One internal examiner. External examiners are used regularly.

Per Ola Rønning