KJVE3510 Analytical Chemistry II Course description

The students shall acquire in-depth knowledge of important instrumental analysis methods, sampling and sample preparation in analytical chemistry. The students shall also receive training in principles for method development and method validation, quality control and quality assurance in a chemical laboratory.

The course builds on KJPE1300 General Chemistry, KJFP1400 Organic Chemistry, KJM1500 Physical Chemistry, and KJM2300 Analytical Chemistry I. It is recommended that students taking the course have passed the exam in KJM2300 Analytical Chemistry I.

Approved laboratory course in KJTS2100 Chemical Engineering, KJM2300 Analytical Chemistry I and KJM2400 Biochemistry and Microbiology, or corresponding qualifications.

After completing the course, the student is expected to have achieved the following learning outcomes defined in terms of knowledge, skills and general competence:

Knowledge

The student is capable of explaining:

• theoretical and practical principles for the use of chromatographic techniques, with the emphasis on gas chromatography and liquid chromatography
• theoretical and practical principles for capillary electrophoresis
• the theory and functioning of mass spectrometry, basic interpretation of mass spectra
• general sampling principles and practical techniques for sampling gas, liquid and solids
• important sample preparation techniques for chromatographic analysis, including extraction, preparative chromatography and concentration techniques
• principles for method development, optimisation and method validation for chromatography
• principles for quality control and quality assurance in a chemical laboratory
• the importance of planning and preparing laboratory work.

Skills

The student is capable of:

• performing quantitative analyses in accordance with standardized operating procedures
• calibrating and adjusting common measurement instruments
• choosing the appropriate laboratory equipment and using it correctly
• making their own assessment in the choice of sample preparation and analysis techniques
• preparing calibration standards and generating calibration curves that meet the necessary quality requirements
• reading scientific articles and searching in journal databases to find relevant literature in connection with method development and implementation
• using statistical methods to interpret and quality check measurement results
• determining the identity of unknown analytes based on chromatographic and mass spectrometry analyses

General competence

The student:

• has insight into the application and limitations of common chromatographic, capillary electrophoretic and mass spectrometry analysis techniques
• has insight into requirements for good laboratory practice
• has knowledge of the principles for developing new analysis methods, including sampling, sample preparation, qualitative and quantitative analysis
• has knowledge of how accuracy and precision in measurement results are affected by sources of error and uncertainty in instrumentation, procedures and work techniques
• has a background for understanding developments and future perspectives in the field
• is capable of communicating their own results orally and in writing

The teaching is organised as lectures, exercises and laboratory instruction.

The following coursework is compulsory and must be approved before the student can sit the exam:

• 8-day compulsory laboratory course with 2 written assignments (groups of 2-4 students, 10-20 pages per assignment)
• 1-day compulsory workshop in bioanalytical metods (4 h laboratory work, groups of 8-10 students, no written assignments).
• Some exercise sessions related to the laboratory course will be compulsory. These sessions will be announced separately
• Before a laboratory exercise starts, a supervision meeting shall be held where the students present a written plan for how to perform the exercise.
• A written collaboration assignment in the form of a case study (groups of 2-4 students). Oral presentation of the solution to the case assignment (4-6 hours).

Exam form: Individual written exam, 3 hours

The exam result can be appealed.

In the event of a resit or rescheduled exam, oral examination may be used instead of written. If oral exams are used for resit and rescheduled exams, the exam result cannot be appealed.

A handheld calculator that cannot be used for wireless communication or to perform symbolic calculations. If the calculator's internal memory can store data, the memory must be deleted before the exam. Random checks may be carried out.

A grade scale with grades from A to E for pass (with A being the highest grade and E being the lowest pass grade) and F for fail is used for the final assessment.

One internal examiner. External examiners are used regularly.